Bio-specific immobilization of enzymes on electrospun PHB nanofibers

Enzyme immobilization provides substantial advantages in terms of improving the efficiency enzymatic process as well enhancing reusability enzymes. Phasins (PhaPs) are naturally occurring polyhydroxyalkanoate (PHA)-binding proteins, and thus can potentially be used a fusion partner for oriented enzymes onto PHA supports. However, presently available granular supports have low surface-area-to-volume ratio limited configurational flexibility reactions. In this study, we explored use electrospun polyhydroxybutyrate (PHB) nanofibers an alternative support high density PhaP-fused lipase. As envisioned, PHB could anchor 120-fold more enzyme than granules same weight. Furthermore, immobilized exhibited markedly higher stability activity compared to when on conventional Our approach combines advantageous features nanofibrous material specificity biomolecular interaction efficient enzymes, which widely adopted development various processes.